Recombinant Human Apolipoprotein A-II, His (HEK293-expressed) 在 HEK293 細(xì)胞中表達(dá)，N 端帶有一個(gè) His 標(biāo)簽。Apolipoprotein A-II (ApoA-II) 與 HDL 代謝的許多方面有關(guān)，并且 apoA-II 過表達(dá)產(chǎn)生促動(dòng)脈粥樣硬化表型。
Synonyms
rHuApolipoprotein A-II, His; ApoA2; Apolipoprotein A-II
Species
HumanSource
HEK 293 Accession
P02652 Gene ID
336 Molecular Weight
10-15 kDa AA Sequence
QAKEPCVESL VSQYFQTVTD YGKDLMEKVK SPELQAEAKS YFEKSKEQLT PLIKKAGTEL VNFLSYFVEL GTQPATQHHH HHH Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Apolipoprotein A-II, His (HEK293-expressed) (rHuApolipoprotein A-II, His) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Apolipoprotein A-II, His (HEK293-expressed) (rHuApolipoprotein A-II, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant apoA-II is comparable to the plasma form in its ability to bind and reorganize lipid and promote cholesterol efflux from macrophages via the ATP binding cassette transporter A1. Synthesized primarily in the liver, apoA-II is a 77 amino acid protein with a single cysteine at position. In healthy humans, apoA-II exists almost entirely as a disulfide-linked homodimer (molecular mass, 17.4 kDa), though it can be occasionally found as a heterodimer with other Cys-containing proteins[1]. |
