Recombinant Rat Thrombopoietin, His (CHO-expressed) 是一種在 CHO 細(xì)胞中產(chǎn)生的帶有標(biāo)簽的重組大鼠血小板生成素 (TPO)。Recombinant Rat Thrombopoietin 是巨核細(xì)胞生成的主要細(xì)胞因子調(diào)節(jié)因子。
Synonyms
rRtThpo, His; C-mpl ligand; MGDF ; 重組大鼠血小板生成素，His 標(biāo)簽
Species
RatSource
CHO Accession
P49745 Gene ID
81811 Molecular Weight
Approximately 22 kDa AA Sequence
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKFHHHHHH Biological Activity
The ED50 is <0.2 ng/mL as measured by human MO7e cells, corresponding to a specific activity of >5 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Thrombopoietin, His (CHO-expressed) (rRtThpo, His) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Rat Thrombopoietin, His (CHO-expressed) (rRtThpo, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Thrombopoietin (TPO) is the master cytokine regulator of megakaryopoiesis. In addition to regulation of megakaryocyte and platelet number, TPO is important for maintaining proper hematopoietic stem cell (HSC) function. TPO produced mainly in the liver, stimulates both megakaryocyte colony formation and enhances megakaryocyte maturation in the bone marrow leading to increased platelet production. TPO binds to its receptor, TPOR/MPL, which is expressed on hematopoietic stem cells (HSCs), megakaryocytes and platelets. Circulating TPO concentrations are controlled by MPL via receptor-mediated internalization and degradation, and in part by sensing of senescent platelets by the Ashwell-Morell receptor in the liver[1]. |
