Recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed) 是一類 I 型跨膜糖蛋白，與 I 型跨膜信號轉導蛋白gp130 (CD130)形成復合物，調控 IL-6 的生物學活性。
Synonyms
rHuIL-6 Receptor α; gp80; CD126
Species
HumanSource
Sf9 insect cells Accession
P08887 Gene ID
3570 Molecular Weight
Approximately 50 kDa AA Sequence
HHHHHHDDDD KLAPRRCPAQ EVARGVLTSL PGDSVTLTCP GVEPEDNATV HWVLRKPAAG SHPSRWAGMG RRLLLRSVQL HDSGNYSCYR AGRPAGTVHL LVDVPPEEPQ LSCFRKSPLS NVVCEWGPRS TPSLTTKAVL LVRKFQNSPA EDFQEPCQYS QESQKFSCQL AVPEGDSSFY IVSMCVASSV GSKFSKTQTF QGCGILQPDP PANITVTAVA RNPRWLSVTW QDPHSWNSSF YRLRFELRYR AERSKTFTTW MVKDLQHHCV IHDAWSGLRH VVQLRAQEEF GQGEWSEWSP EAMGTPWTES RSPPAENEVS TPMQALTTNK DDDNILFRDS ANATSLPVQD Biological Activity
The ED50 is <50 ng/mL as measured by M1 cells, corresponding to a specific activity of >2 × 104 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed) (rHuIL-6 Receptor α) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed) (rHuIL-6 Receptor α) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
It is found that soluble IL-6 receptor alpha-subunit (IL-6Ralpha), with their constitutive IL-6 synthesis, stimulated endothelial cells to synthesize E-selectin, intracellular adhesion molecule-1, vascular cellular adhesion molecule-1, IL-6, and IL-8, and to bind neutrophils. Neutrophils express significant amounts of IL-6Ralpha and upon stimulation shed it: this material activates endothelial cells through a newly constituted IL-6 receptor. Retrograde signaling from PMN activated in the extravascular compartment to surrounding endothelial cells will recruit more and a wider variety of leukocytes. The limiting signal is a soluble receptor, not a cytokine[1]. |
