Recombinant Human Insulin-like growth factor-binding protein 2, His (HEK-expressed) 是高親和力結合蛋白家族成員 (IGFBP1-6)，在中樞神經(jīng)系統(tǒng)胰島素樣生長因子 (IGF) 調控中發(fā)揮調控作用。
Synonyms
rHuIGF-BP-2, His; IBP-2; IGFBP-2; BP2 ; 重組人胰島素樣生長因子結合蛋白 2 His (HEK 細胞表達)
Species
HumanSource
HEK 293 Accession
P18065 Gene ID
3485 Molecular Weight
6-20 kDa AA Sequence
FRCPPCTPER LAACGPPPVA PPAAVAAVAG GARMPCAELV REPGCGCCSV CARLEGEACG VYTPRCGQGL RCYPHPGSEL PLQALVMGEG TCEKRRDAEY GASPEQVADN GDDHSEGGLV ENHVDSTMNM LGGGGSAGRK PLKSGMKELA VFREKVTEQH RQMGKGGKHH LGLEEPKKLR PPPARTPCQQ ELDQVLERIS TMRLPDERGP LEHLYSLHIP NCDKHGLYNL KQCKMSLNGQ RGECWCVNPN TGKLIQGAPT IRGDPECHLF YNEQQEARGV HTQRMQHHHH HH Biological Activity
The ED50 is <2 μg/mL as measured by FDC-P1 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Insulin-like growth factor-binding protein 2, His (HEK-expressed) (rHuIGF-BP-2, His) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Insulin-like growth factor-binding protein 2, His (HEK-expressed) (rHuIGF-BP-2, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Insulin-like Growth Factor Binding Protein 2 (IGFBP2) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system[1]. Insulin-like Growth Factor-Binding Proteins (IGFBPs) modulate the actions of secreted insulin-like growth facts (IGFs) by binding to them and increase the IGF halflife in the extracellular milieu and circulation by sequestering them in this bound form. IGFBPs either enhance or inhibit IGF actions on target cells; the individual IGFBPs either inhibit or potentiate IGF effects on osteoblasts in bone. Human Insulin-like Growth Factor-Binding Protein 5 (rhIGFBP-5) is a unique and most abundant IGFBP stored in bone, having a high specific binding affinity for hydroxyapatite and extracellular matrix proteins, therefore fixing it and its bound IGFs within bone. rhIGFBP-5 consistently stimulates osteoblast cell proliferation in vitro, thus increasing the number of osteoblasts[2]. |
