Recombinant Human Fractalkine/CX3CL1 (CHO-expressed) 是 CX3C 趨化因子家族的成員，與多種腫瘤有關(guān)，促進(jìn)腫瘤進(jìn)展。
Synonyms
rHuFractalkine/CX3CL1; Fractalkine; CX3CL1; FKN; SCYD1; Neurotactin; NTT ; 重組人趨化因子/CX3CL1 (CHO 細(xì)胞表達(dá))
Species
HumanSource
CHO Accession
P78423 Gene ID
6376 Molecular Weight
50-75 kDa AA Sequence
QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNGGTFE KQIGEVKPRT TPAAGGMDES VVLEPEATGE SSSLEPTPSS QEAQRALGTS PELPTGVTGS SGTRLPPTPK AQDGGPVGTE LFRVPPVSTA ATWQSSAPHQ PGPSLWAEAK TSEAPSTQDP STQASTASSP APEENAPSEG QRVWGQGQSP RPENSLEREE MGPVPAHTDA FQDWGPGSMA HVSVVPVSSE GTPSREPVAS GSWTPKAEEP IHATMDPQRL GVLITPVPDA QAATR Biological Activity
The EC50 is <1.5 μg/mL as measured on Ca2+ mobilization assay in CHO-K1/Gα15/hCX3CR1 cells (human Gα15 and hCX3CR1 stably expressed in CHO-K1 cells). Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Fractalkine/CX3CL1 (CHO-expressed) (rHuFractalkine/CX3CL1) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Fractalkine/CX3CL1 (CHO-expressed) (rHuFractalkine/CX3CL1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Fractalkine is the only member of the CX3C chemokine family so far. It is expressed in many cell types such as hematopoietic cells, endothelial cells, epithelial cells, smooth muscle cells, dendritic cells, neurons, and fibroblasts. Fractalkine exists in two forms that have distinct cellular functions. The soluble form is a chemoattractant for cells expressing the CX3CR1 receptor. The membrane-bound form functions as an adhesion molecule that promotes adhesion and migration[1]. |
