| Recombinant Human Desert Hedgehog 是在 E. coli 中產(chǎn)生的一種非糖基化肽鏈。Desert hedgehog protein (DHH) 是 Hedgehog 家族成員，可編碼信號(hào)分子，在調(diào)節(jié)形態(tài)發(fā)生中起重要作用。 Synonyms
rHuDHH; DHH; HHG-3
Species
Human Source
E. coli Accession
O43323 Gene ID
50846 Molecular Weight
Approximately 20 kDa AA Sequence
IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG Biological Activity
The ED50 is <10 μg/mL as measured by CCL-226 cells, corresponding to a specific activity of >100 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Desert Hedgehog (rHuDHH) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized Recombinant Human Desert Hedgehog (rHuDHH) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. The receptors PTCH1 and PTCH2 are highly conserved mediators of hedgehog signalling in both the developing and adult marsupial gonads. Together these findings indicate DHH is an essential therian mammal-specific morphogen in gonadal development and gametogenesis[1]. |
