Recombinant Human SPARC 是Ca+2 結(jié)合的糖蛋白，與形態(tài)發(fā)生、重塑、細(xì)胞遷移和增值相關(guān)。
Synonyms
rHuSPARC; BM-40; Osteonectin ; 重組人的富含半胱氨酸的酸性蛋白
Species
HumanSource
E. coli Accession
P09486 Gene ID
6678 Molecular Weight
Approximately 36.1 kDa AA Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FE Biological Activity
The ED50 is <3.0 μg/mL as measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells, corresponding to a specific activity of >333 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human SPARC (rHuSPARC) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Human SPARC (rHuSPARC) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
SPARC (secreted protein, acidic and rich in cysteine) is the founding member of a family of secreted matricellular proteins with similar domain structure. SPARC shows context specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF[1][2]. |
