Recombinant Rat Macrophage-Colony Stimulating Factor (CHO-expressed)是一種促炎細(xì)胞因子，其與其受體CSF1R結(jié)合，參與單核細(xì)胞/巨噬細(xì)胞譜系細(xì)胞的發(fā)育和增殖，并參與破骨細(xì)胞的誘導(dǎo)。
Synonyms
rRtM-CSF; CSF1; MCSF ; 重組大鼠巨噬細(xì)胞集落刺激因子 (CHO表達(dá))
Species
RatSource
CHO Accession
Q8JZQ0 Gene ID
78965 Molecular Weight
32-40 kDa AA Sequence
EVSEHCSHMI GNGHLQILQQ LIDSQMETAC LIEYKFVDQE QLDDPVCYLK KAFVLVQVII EETMRFKDNT PNANATERLQ ELSMKLNSCF IKDYKEQNEA CVQTYKESPL RLLEKIKNFF NETKNFLEKD WNIFSKNCND SFAKCSSRDV VTKP Biological Activity
The ED50 is <2.5 ng/mL as measured by murine M-NFS-60 cells, corresponding to a specific activity of >4.0 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Macrophage-Colony Stimulating Factor (CHO-expressed) (rRtM-CSF) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Rat Macrophage-Colony Stimulating Factor (CHO-expressed) (rRtM-CSF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Macrophage Colony Stimulating Factor (M-CSF) is a pro-inflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms- as a secreted glycoprotein, a cell-surface protein and a proteoglycan[1]. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis[2]. |
