Recombinant Rat Leptin是脂肪細(xì)胞分泌的一種激素，通過抑制攝食和增加產(chǎn)熱來幫助調(diào)節(jié)能量平衡。
Synonyms
rRtLeptin; Obesity protein (OB) ; 重組大鼠瘦素
Species
RatSource
E. coli Accession
P50596 Gene ID
25608 Molecular Weight
Approximately 16.3 kDa AA Sequence
MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC Biological Activity
The ED50 is <10 μg/mL as measured by LoVo cells, corresponding to a specific activity of >100 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Leptin (rRtLeptin) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Leptin (rRtLeptin) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
A sensor (leptin production by adipose cells) monitors the size of the adipose tissue mass. Hypothalamic centers receive and integrate the intensity of the leptin signal through leptin receptors (LRb). Effector systems, including the sympathetic nervous system, control the two main determinants of energy balance-energy intake and energy expenditure[1]. Recessive mutations in the leptingene are associated with massive obesity in mice and humans, establishing a genetic basis for obesity. Leptin circulates in blood and acts on the brain to regulate food intake and energy expenditure. When fat mass falls, plasma leptin levels fall, stimulating appetite and suppressing energy expenditure until fat mass is restored. When fat mass increases, leptin levels increase, suppressing appetite until weight is lost. This system maintains homeostatic control of adipose tissue mass[2]. |
