Recombinant Mouse Granzyme B (CHO-expressed) 在細胞凋亡中發(fā)揮重要作用，通過刺激細胞因子釋放參與炎癥誘導，并參與細胞外基質重構。
Synonyms
rMuGranzyme B; CTLA-1; CCP1; Fragmentin-2; Gzmb ; 重組小鼠顆粒酶 B (CHO 細胞表達)
Species
MouseSource
CHO Accession
P04187 Gene ID
14939 Molecular Weight
Approximately 32 kDa AA Sequence
IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS Biological Activity
The specific activity is >1000 nM/min per μg of enzyme, measured by its ability to cleave a chromogenic peptide substrate (Ac-IEPD-pNA). Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Granzyme B (CHO-expressed) (rMuGranzyme B) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Mouse Granzyme B (CHO-expressed) (rMuGranzyme B) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Granzyme B plays essential roles in mammalian apoptosis[1]. Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs), natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling[2]. |
