Recombinant Rhesus Macaque Granulocyte Macrophage Colony Stimulating Factor 是一種細(xì)胞因子，作用多種類型的細(xì)胞，尤其是巨噬細(xì)胞和嗜酸性粒細(xì)胞。
Synonyms
rRhGM-CSF; CSF2; Colony stimulating factor 2 ; 重組恒河猴粒細(xì)胞巨噬細(xì)胞集落刺激因子
Species
Rhesus MacaqueSource
E. coli Accession
Q9GL44 Gene ID
574371 Molecular Weight
Approximately 14.4 kDa AA Sequence
APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE Biological Activity
The ED50 is <0.1 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of >1.0 × 107 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rhesus Macaque Granulocyte Macrophage Colony Stimulating Factor (rRhGM-CSF) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Rhesus Macaque Granulocyte Macrophage Colony Stimulating Factor (rRhGM-CSF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a monomeric glycoprotein that functions as a cytokine —it is a white blood cell growth factor[1]. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Monocytes exit the circulation and migrate into tissue, whereupon they mature into macrophages and dendritic cells. GM-CSF also has some effects on mature cells of the immune system. These include, for example, inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface[2]. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract[3]. |
