Recombinant Rat Ciliary Neurotrophic Factor是一種多能性神經(jīng)營(yíng)養(yǎng)因子，可保護(hù)視網(wǎng)膜錐細(xì)胞和視桿細(xì)胞的光感受器。
Synonyms
rRtCNTF; Ciliary Neurotrophic Factor
Species
RatSource
E. coli Accession
P20294 Gene ID
25707 Molecular Weight
Approximately 22.9 kDa AA Sequence
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM Biological Activity
The ED50 is <30 ng/mL as measured by its ability to induce alkaline phosphatase production by TF-1 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Ciliary Neurotrophic Factor (rRtCNTF) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Ciliary Neurotrophic Factor (rRtCNTF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Ciliary Neurotrophic Factor (CNTF) is an extracellular signaling protein in the neuroretinal and the interphotoreceptor matrix, which is associated with the membranes of the RPE, Muller and photoreceptor cells[1]. CNTF has neuroprotective effects on a variety of central and also peripheral nervous system neurons. Because it promotes differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes under in vitro conditions and thus improves remyelination. Importantly, it also increases the survival of mature oligodendrocytes[2]. |
