Recombinant Human BCA-1/CXCL13 是一種有效的、選擇性B淋巴細(xì)胞引誘劑，也是一種B細(xì)胞定位趨化因子。
Synonyms
rHuBCA-1/CXCL13; C-X-C motif chemokine 13; BCA1; BLC; SCYB13 ; 重組人源 CXC 趨化因子13 (CXCL13)
Species
HumanSource
E. coli Accession
O43927 Gene ID
10563 Molecular Weight
Approximately 10.3 kDa AA Sequence
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP Biological Activity
Full biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/mL. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 100 mM NaCl. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human BCA-1/CXCL13 (rHuBCA-1/CXCL13) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Human BCA-1/CXCL13 (rHuBCA-1/CXCL13) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Human BCA-1/CXCL13 is identified as an efficacious and selective attractant for B lymphocytes, also acts as a B cell homing chemokine. Human BCA-1 competes with radiolabeled interferon gamma (IFN-γ) inducible protein 10 (IP-10) for binding to the human CXCR3 receptor. Furthermore, human BCA-1 is an efficacious attractant for human CXCR3 transfected cells; BCA-1-induced chemotaxis is inhibited by a monoclonal antibody against human CXCR3. Indeed, BCA-1 attenuates the calcium flux induced by IP-10. In addition, human BCA-1 is an agonist in stimulating GTPγS binding[1] |
