Recombinant Human BDNF/Brain-derived neurotrophic factor (E.coli) 是一種神經(jīng)營(yíng)養(yǎng)因子，能夠與 TrkB 受體結(jié)合，調(diào)節(jié)神經(jīng)發(fā)育過(guò)程，包括神經(jīng)元的存活，分化，及突觸可塑性。
Synonyms
rHuBDNF/Brain-derived neurotrophic factor; Abrineurin ; 重組人腦源性神經(jīng)營(yíng)養(yǎng)因子 (E.coli 表達(dá))
Species
HumanSource
E. coli Accession
P23560 Gene ID
627 Molecular Weight
Approximately 13 kDa AA Sequence
HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR Biological Activity
The ED50 is <5 μg/ml measured by its ability to bind Human TrkB in functional ELISA. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, 250 mM NaCl, pH 7.2. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human BDNF/Brain-derived neurotrophic factor (rHuBDNF/Brain-derived neurotrophic factor) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human BDNF/Brain-derived neurotrophic factor (rHuBDNF/Brain-derived neurotrophic factor) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human BDNF/Brain-derived neurotrophic factor is a neurotrophin binding to the high-affinity tropomyosin-related receptor kinase B (TrkB) receptor to regulate neurodevelopmental processes, including neuronal survival, neuronal differentiation, and synaptic plasticity[1]. Brain-derived neurotrophic factor (BDNF) is one of the most abundant neurotrophins in the mammalian central nervous system that promotes neuronal survival and differentiation. BDNF plays crucial roles in the cardiovascular system[2]. |
