Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed) 是一種很強的促炎性細胞因子，在免疫系統(tǒng)中具有調(diào)節(jié)功能，同時在炎癥，細胞增殖、分化及凋亡過程中發(fā)揮著重要作用。
Synonyms
rRtTNF-α/TNFSF2; TNF-alpha; Cachectin; DIF; TNFA; Differentiation-inducing factor ; 重組大鼠腫瘤壞死因子-α/TNFSF2 (P.pastoris 表達)
Species
RatSource
P. pastoris Accession
P16599 Gene ID
24835 Molecular Weight
Approximately 17.4 kDa AA Sequence
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL Biological Activity
The ED50 is <50 pg/mL as measured by mouse L-929 cells, corresponding to a specific activity of >2.0 × 107 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed) (rRtTNF-α/TNFSF2) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed) (rRtTNF-α/TNFSF2) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Tumor necrosis factor α (TNF-α, also known as cachectin) is a strong pro-inflammatory cytokine playing an important role in the immune system during inflammation, cell proliferation, differentiation and apoptosis. TNF-α is type II transmembrane protein that can be expressed in both membrane-bound and secreted forms. Receptors for TNF-a are part of TNF receptor (TNFR) superfamily, with characteristic intracellular segment, a transmembrane domain, and an extracellular ligand-binding domain. They are type I transmembrane glycoproteins characterized by a cysteinerich motif (up to 40 aminoacids) repeated 2-6 times in the extracellular N-terminal domain[1]. |
