Recombinant Rat Interleukin-1 beta 是一種免疫調(diào)節(jié)細(xì)胞因子，可介導(dǎo)廣泛的免疫反應(yīng)。
Synonyms
rRtIL-1β; Catabolin; IL1F2; IL-1 beta; IL1B
Species
RatSource
E. coli Accession
Q5BKB0 Gene ID
24494 Molecular Weight
Approximately 17.3 kDa AA Sequence
VPIRQLHCRL RDEQQKCLVL SDPCELKALH LNGQNISQQV VFSMSFVQGE TSNDKIPVAL GLKGLNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKTKV EFESAQFPNW YISTSQAEHR PVFLGNSNGR DIVDFTMEPV SS Biological Activity
The ED50 is <10 pg/mL as measured by mouse D10S cells, corresponding to a specific activity of >1.0 × 108 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Interleukin-1 beta (rRtIL-1β) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Interleukin-1 beta (rRtIL-1β) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine, produced in response to inflammatory agents by a variety of cells, including, monocytes, macrophages, and dendritic cells (DCs). Recombinant rat IL-1β and IL-6 synergistically enhance C3 mRNA levels and complement component C3 secretion by H-35 rat hepatoma cells. In rat hepatoma H-35 cells, recombinant rat IL-1β, IL-6, and IFN-γ increase the quantity of C3 mRNA and secreted C3 protein, an observation consistent with reports that C3 is an acute phase protein in the rat[1]. |
