Recombinant Mouse Interleukin-11 (HEK293-expressed) 是一種 HEK293 細胞來源的多效細胞因子，在許多細胞類型中調(diào)節(jié)細胞周期，侵襲和遷移。
Synonyms
rMuIL-11; Adipogenesis inhibitory factor; AGIF
Species
MouseSource
HEK 293 Accession
P47873 Gene ID
16156 Molecular Weight
Approximately 21 kDa AA Sequence
GPPAGSPRVS SDPRADLDSA VLLTRSLLAD TRQLAAQMRD KFPADGDHSL DSLPTLAMSA GTLGSLQLPG VLTRLRVDLM SYLRHVQWLR RAGGPSLKTL EPELGALQAR LERLLRRLQL LMSRLALPQA APDQPVIPLG PPASAWGSIR AAHAILGGLH LTLDWAVRGL LLLKTRL Biological Activity
The ED50 is <3 ng/mL as measured by T11 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Interleukin-11 (HEK293-expressed) (rMuIL-11) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Murine Interleukin-11 (HEK293-expressed) (rMuIL-11) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-11 (IL-11) is a pleiotropic cytokine that regulates cell cycle, invasion, and migration in numerous cell types, all roles critical to placental development. IL-11 is a member of the IL-6-type cytokines and signals via the IL11 receptor (R) α chain and signal transducer gp130 to activate the Janus kinase (JAK)/Signal transducers and activators of transcription (STAT)3 pathway in human endometrium and primary human extravillous trophoblast (EVT) . IL-11 is required for decidualization in humans and mice. IL11 levels are elevated in preeclampsia (PE) decidual tissue. L-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell[1]. |
