Recombinant Human Fibroblast Growth Factor 23 是一種獨(dú)特的成纖維細(xì)胞生長(zhǎng)因子 (FGF)，它是一種激素，通過(guò)輔因子如 α-Klotho 與 FGF 受體結(jié)合發(fā)揮作用，在軟骨形成過(guò)程中誘導(dǎo)肥大和礦化的產(chǎn)生。
Synonyms
rHuFGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor ; 重組人成纖維細(xì)胞生長(zhǎng)因子 23
Species
HumanSource
E. coli Accession
Q9GZV9 Gene ID
8074 Molecular Weight
Approximately 25.3 kDa AA Sequence
YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI Biological Activity
The ED50 is <0.5 μg/mL as measured by FGF-receptors transfected BaF3 cells, corresponding to a specific activity of >2.0 × 103 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Fibroblast Growth Factor 23 (rHuFGF-23) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA. Storage & Stability
Lyophilized recombinant Human Fibroblast Growth Factor 23 (rHuFGF-23) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Fibroblast Growth Factor 23 is a unique FGF subfamily member, acts as a a hormone and requires a cofactor to signal through canonical FGFR, and induces hypertrophy and mineralization during chondrogenesis[1]. FGF23 is a secreted, circulating protein of 32 kDa that is predominantly produced by osteocytesin bone and functions in distant target organs in an endocrine fashion. FGF23 generally requires an FGF receptor (FGFR) and α-Klotho to evoke its signaling. GF23 binds to an FGFR-α-Klotho complex and induces the phosphorylation of FGFR and the expression of early growth response-1 (Egr-1), which encodes a transcription factor[2]. |
